4.4.23-Health-Blainey

Conference Video|Duration: 32:23
April 4, 2023
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    Today, we investigate and interpret the biology of human cells and their roles in disease through a “cell state” paradigm. We often parameterize cell state via high-content, single-cell molecular profiling. The need to expand this paradigm to include the response of cells to perturbations, the local context of the cells, and additional profiling data modes is clear.  Less clear, however, is how we should navigate the expanding panoply of technologies to generate interpretable data at large scales in the most relevant human biological model systems in ways that are relevant to disease. Many available technologies are currently hobbled by high costs or limited applicability to sample types of interest. My group has developed optical pooled screening (OPS), an in situ genomic approach for profile-based screening of the effects of genomic perturbations in human cells at massive scales. Image-based phenotyping provides access to valuable cellular phenotypes complementary to sequencing-based methods and currently provides routine throughputs of tens of millions of cells at modest cost. I will describe the OPS technology and review several recent large-scale applications. I will also speak to ongoing efforts to expand the performance and applicability of OPS in collaboration with industrial partners.
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  • Video details
    Today, we investigate and interpret the biology of human cells and their roles in disease through a “cell state” paradigm. We often parameterize cell state via high-content, single-cell molecular profiling. The need to expand this paradigm to include the response of cells to perturbations, the local context of the cells, and additional profiling data modes is clear.  Less clear, however, is how we should navigate the expanding panoply of technologies to generate interpretable data at large scales in the most relevant human biological model systems in ways that are relevant to disease. Many available technologies are currently hobbled by high costs or limited applicability to sample types of interest. My group has developed optical pooled screening (OPS), an in situ genomic approach for profile-based screening of the effects of genomic perturbations in human cells at massive scales. Image-based phenotyping provides access to valuable cellular phenotypes complementary to sequencing-based methods and currently provides routine throughputs of tens of millions of cells at modest cost. I will describe the OPS technology and review several recent large-scale applications. I will also speak to ongoing efforts to expand the performance and applicability of OPS in collaboration with industrial partners.
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