HiExM: High-Throughput Super-Resolution Imaging Enables Unique Insights for Drug Discovery John Day Doctoral Candidate, Prof. Laurie Boyer’s Research Group
High-throughput imaging is one of the primary methods used in drug screening and discovery. Current methods focus on maximizing throughput with a trade-off of reduced image resolution. To address this gap, we developed High-throughput Expansion Microscopy (HiExM), a robust platform that leverages expansion microscopy to achieve nanoscale image resolution. Our method is compatible with standard culture ware, commonly employed immunostaining practices, and existing high-content confocal microscopes, making it widely adoptable. We use HiExM to access information about intracellular localization, organelle morphology, and toxicity of therapeutics that would otherwise be inaccessible.