Entry Date:
October 22, 2008

Microtools for Profiling Immune Responses

Principal Investigator J Love

Samples from human patients used for clinical research are often limited in size and availability, and cells of particular interest are usually rare and have short viabilities. Therefore, a critical challenge for advancing clinical immunology is the development of new technologies that make it possible to collect quantitative data for a large number of individual primary cells (104-106) in one experiment that is equivalent to a series of independent assays (flow cytometry, ELISPOT, and genotyping). Such technologies would enable the construction of highly correlated datasets that detail the state of the cellular immune system; such profiles would (1) facilitate the development of new antimicrobial immunotherapies and (2) improve immunological monitoring for diagnosing a disease and analyzing the efficacy of immunotherapies over time.