Principal Investigator Bruce Tidor
Co-investigators Michael Yaffe , Douglas Lauffenburger , Leona Samson
Project Website http://web.mit.edu/icbp/research/dna_repair.html
Specific aims of this project are:
Aim 1: Develop and Implement High-Throughput Kinase Assays for Monitoring the Signaling Response to DNA Damage.
Aim 2: Monitor Kinase Localization, Cell Cycle Progression, and Apoptosis in Response to DNA Damage.
Aim 3: Construct a Quantitative Predictive Systems Biology Model for Protein Kinase Network Responses During DNA Damage Signaling.
Aim 4: Utilize Knock-Out Mutant Analysis, shRNA Technologies, and Genetic Engineering in Cells and Mice to Test Hypotheses Generated from the Model and to Further Probe DNA Damage Signaling Networks.
Aim 5: Discovery of New Kinase Substrates that Play Key Roles in the DNA Damage Response.