Principal Investigator Tania Baker
Impact of Clp-Mediated Degradation of the Bacterial Proteome
We have developed a method to trap proteins in vivo that are substrates for ClpXP or the related enzyme ClpAP. This allowed the identification of more than 60 new ClpXP substrates in cells growing under nonstressed conditions; numerous additional proteins were found when cells were exposed to a DNA-damaging agent. The proteins include transcription factors, metabolic enzymes, and proteins involved in the starvation and oxidative stress responses. We are continuing to use this approach to understand the global role of proteolysis in resculpting the proteome in response to changing environmental conditions.