Prof. Jongyoon Han
Primary DLC
Areas of Interest and Expertise
Separation and Analysis of Biomolecules
BioMEMS
Cell Sorting
Inertial Microfluidics
Microfluidics and Nanofluidics
Electrokinetics and Its Applications
Biological and Physiological Transport Phenomena
Neural Prosthetics and Neurotechnology
Desalination and Chemical Separation
Application of Micro-Nanofabrication to Biological Problems Biological MEMS, Biomolecular Analysis Biosensing
Proteomic Sample Preparation Electrochemical Neuroengineering
Research Summary
(1) Biomolecule separation using nanofluidic molecular sieve: Currently, most of biomolecule purification and separation uses random nanoporous materials as molecular sieving matrix. We are developing MEMS(Micro-Electro-Mechanical System)-based nanofluidic molecular sieves that can filter and separate various biomolecules based on their size or charge density. Unlike polymeric gels or nanoporous molecular filters, nanofluidic molecular sieves and filters could be engineered to have precise physical and chemical characteristics, therefore can have higher separation efficiency and selectivity.
(2) Biomolecule and cell concentration / sensing using ion selective membranes: Ion selective membranes, such as nanofluidic channels and charged gels, can create the phenomenon of ion concentration polarization (ion depletion), which moves around ions and charged molecules in a controllable manner in a microfluidic system. We have developed various biomolecule and cell concentration devices using this phenoemenon, enabling higher detection sensitivities for immuoassays, enzyme activity assays, and cell based assays.
(3) Small scale seawater desalination: Using the ion concentration polarization, we have developed an energy efficient but scalable seawater desalination and water purification system. The separation mechanism is appliable to a broad class of contaminants, including salts, heavy metal ions, virus and bacteria particles, and other colloid in a single step operation. The energy efficiency of this desalination process is comparable to the current state-of-the-art large scale reverse osmosis, but the technology is scalable and miniaturiazable, ideally suited for portable, self-powered water purification, for remote and disaster relief applications.
(4) Electrochemical modulation of nerve cells using ion selective membranes: Ion selective membranes can be used to convert electric signals into electrochemical one, by modulating ion concentrations near the nerve cells. We are currently studying the method of locally modulating various ion concentrations near the nerve cells, in order to change the nerve cells' excitability on demand. This could have broad implacations in neural prosthetics engineering, by facilitating low-current nerve stimulation and inactivation for the next generation neural prosthetics.
(summary updated 10/2012)
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Projects
September 3, 2021
Off-Grid Portable Ion Concentration Polarization Desalination Unit
Principal Investigator Jongyoon Han
January 24, 2019Department of Electrical Engineering and Computer ScienceCurrent-Induced Domain Wall Motion in Compensated Ferrimagnets
Principal Investigator Jongyoon Han
January 22, 2019Department of Electrical Engineering and Computer ScienceIon Concentration Polarization Desalination Using Return Flow System
Principal Investigator Jongyoon Han
Room Temperature Spin-Orbit Torque Switching Induced by a Topological Insulator
Principal Investigator Jongyoon Han
September 10, 2015Department of Electrical Engineering and Computer ScienceNext Generation Brine Desalination and Management for Efficiency, Reliability, and Sustainability
Principal Investigator Jongyoon Han
April 22, 2010Department of Electrical Engineering and Computer ScienceNonlinear Electrokinetics
Principal Investigator Jongyoon Han
April 22, 2010Department of Electrical Engineering and Computer ScienceContinuous Biomolecular Fractionation
Principal Investigator Jongyoon Han
April 22, 2010Department of Electrical Engineering and Computer ScienceMultiscale Fabrications
Principal Investigator Jongyoon Han
October 1, 2007Department of Electrical Engineering and Computer ScienceA Patterned Anisotropic Nanofilter Array for Continuous-Flow Separation of DNA and Proteins
Principal Investigator Jongyoon Han
September 27, 2007Department of Electrical Engineering and Computer ScienceHigh-Throughput, Continuous-Flow Separation of Biomolecules in a High-Aspect-Ratio Nanofilter Array
Principal Investigator Jongyoon Han
October 17, 2006Department of Electrical Engineering and Computer ScienceBiomolecule Confinement and Preconcentration Using Nanofluidic Filters
Principal Investigator Jongyoon Han
February 21, 2006Department of Electrical Engineering and Computer ScienceNanofluidic Biomolecular Preconcentration and Concentration-Enhanced Assays
Principal Investigator Jongyoon Han
Micro/Nanofluidic BioMEMS Group
Principal Investigator Jongyoon Han
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Video
2024 MIT R&D Conference: Track 4 - Healthcare - Machine-Learning-Guided Quality Control of CAR-T Therapy Product Using Microfluidic Biophysical Cytometry
Machine-Learning-Guided Quality Control of CAR-T Therapy Product Using Microfluidic Biophysical Cytometry
Jongyoon Han
Professor of Electrical Engineering, MIT Electrical Engineering & Computer Science Department
Professor, MIT Biological EngineeringChimeric Antigen Receptor (CAR) T cell therapy has revolutionized cancer care, yet its manufacturing remains challenging due to variability in quality and efficacy. In this talk we introduce a novel microfluidic, label-free cellular biophysical profiling assay that rapidly assesses the functional phenotypes of CAR T cells. Our assay leverages biophysical features such as cell size and deformability to directly correlate with critical functional attributes, including the CD4:CD8 ratio, effector and central memory subtypes, and killing potency. Validated through extensive longitudinal studies across multiple CAR T batches from different donors and culture platforms, this method requires fewer than 10,000 cells and completes profiling within 10 minutes. The assay provides an efficient means to predict CAR T cell quality at critical manufacturing stages, thereby potentially reducing batch failure rates and enhancing therapeutic consistency.
04.10-11.24-HST-Han
Continuous, Intensified Manufacturing of Gene Delivery Vectors Enabled by High-Throughput Microfluidic Systems
4.12.22-Health-Science-Jongyoon-Han
Jongyoon Han
Professor of Electrical Engineering and Professor of Biological Engineering10.2021-Sense.nano-Jongyoon-Han
Jongyoon Han | Professor, MIT Electrical Engineering & Computer Science 10.2021-Sense.nano-Session 2-Physiological-Monitoring-Q-A
Brian Anthony | Associate Director, MIT.nano
Paul Blainey
Associate Professor, MIT Biological Engineering
Ruizhi (Ray) Liao
Postdoctoral Associate, MIT Computer Science & Artificial Intelligence Lab
Jongyoon Han
Professor of Electrical Engineering and Professor of Biological Engineering4.6.21-Water-Industry-Jongyoon-Han
Jongyoon Han
Professor of Electrical Engineering and Professor of Biological Engineering
MIT Department of Electrical Engineering and Computer Science