4.12.22-Health-Science-Deepak-Mishra

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  Organoids are tiny, self-organized three-dimensional (3D) tissue cultures erived from stem cells that allow recapitulation of architecture, composition, and physiology of organs. Organoids may be formed by combining individually differentiated cell populations or via the introduction of external cues to human induced pluripotent stem cells (hiPSCs) to mimic stages of human development. However, such approaches may limit intercellular interactions or faithful mimicry of a mature organ’s structure. We employ synthetic gene circuits to genetically engineer human induced pluripotent stem cells (hIPSCs) with transcriptional and post-transcriptional regulatory elements. These networks govern multi-step differentiation of hiPSCs in a cell-specific, semi-automated manner to form 3D liver organoids. Our recent advances in creation and subsequent use of these organoids in toxicology, drug metabolism, and implantation will be presented and discussed.

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