Microfluidic devices offer unique capabilities to control and manipulate biomolecules and cells, which can be utilized to enhance the efficiency of conventional biomanufacturing processes, as well as to advance novel therapeutic modalities such as cell therapy. In this presentation, I will showcase several examples where high-volume processing microfluidic systems are used for increasing efficiencies for perfusion bioreactor, monitoring product quality in real time at line, and detect low-abundance adventitious agents for enhancing overall safety.