Entry Date:
January 27, 2016

Transcription Factor Organization During Cellular Reprogramming

Principal Investigator David Gifford


The ability to reprogram cells from one type to another presents a powerful tool to diverse areas of research and medicine. We study the interplay between genomic sequence, transcription factor binding, and chromatin architecture during cell state change, with the goal of composing simple mechanistic models that explain transcription factor binding dynamics and which can be used in reprogramming systems. We characterize this interplay using DNase-seq, ChIP-Seq, ChIA-PET, and RNA-seq data, focusing on developmental and stem cell differentiation systems along the pancreatic lineage.