Rapid Assembly and Delivery of Large-Scale Genetic Circuits into Mammalian Cells

This project aims to develop a framework to rapidly and reliably assemble and deliver large scale genetic circuits site-specifically into mammalian cells. The methods we’re developing allow choosing parts from a library of known (sequenced, characterized) mammalian parts and assembling them within a short time frame into large circuits. These circuits are then be integrated in a site specific-manner into the host genome or persist episomally in the nucleus of mammalian cells. Currently we can assemble gene circuits up to 65 kilobases in size with a high efficiency.