Principal Investigator Peter Dedon
Project Website http://dedon.mit.edu/
Research in the Dedon Lab focuses on the chemical biology of nucleic acids in three broad areas: epigenetics, epitranscriptomics, and genetic toxicology.
The Dedon Lab and our many collaborators have developed a variety of analytical and informatic platforms for discovery science in epigenetics and epitranscriptomics in infectious disease, antimicrobial resistance, the human microbiome, and cancer and age-related diseases. One platform coordinates comparative genomics, single-molecule real-time sequencing, and mass spectrometry to discover novel DNA modifications, such as phosphorothioate and 7-deazaguanine modifications in bacterial and bacteriophage genomes. Work in bacteriophage points to a tremendous variety of DNA modifications with implications for biotechnology, synthetic biology, and human health and disease. For example, we have found oxidation-sensitive phosphorothioate DNA modifications in 10-20% of the organisms in the human microbiome.
The Dedon Lab has had a long-standing interest in chemical etiology of human disease, with a focus on the role of inflammation and endogenous DNA damage as drivers of carcinogenesis and age-related diseases. We have developed a variety of analytical tools to interrogate genetic toxicology and endogenous molecular damage, including sensitive chromatography-coupled mass spectrometric methods to quantify dozens of different DNA, RNA and protein damage products. This 'omic approach to quantifying molecular damage allows us to interrogate all types of inflammation chemistry occurring in a tissue, including macrophage-induced oxidative and nitrosative stresses and neutrophil-mediated halogenation chemistry, as well as the indirect damage caused by generation of reactive electrophiles by oxidation of sugars and lipids.
Research in the Dedon Lab is broadly organized into three major areas: (1) The genetic toxicology of inflammation and aging (2) The epitranscriptome in infectious disease and cancer (3) The epigenetics of the human gut microbiome and microbial pathogens