Entry Date:
June 20, 2011

Instant Hemostasis for the Liver

Principal Investigator Rutledge Ellis-Behnke

Rat liver hemostasis. This series of studies is of an adult rat where the skin covering the intra-peritoneal cavity was removed and the liver exposed. The left lateral lobe was sagittally cut completely transecting a portion of the liver lobe. The liver was separated with profuse bleeding. The two halves were allowed to come back together and the bleeding continued. The 1% NHS solution was applied and the extent of the incision was visible under the transparent assembled NHS. Complete hemostasis was achieved in 8.6 seconds, statistically significant when compared to 90.0 seconds when cauterization was applied, or 301.6 seconds if irrigated with saline. Further studies shows a transverse cut to the left lateral lobe in an adult rat. Applying a transverse cut in the lobe we can show the profuse bleeding produced when a major branch of the portal vein is cut, and show extent of cut. Treatment is with self-assembling NHS. There is a complete cessation of bleeding (in 10.3 seconds using 2% concentration; 10.0 seconds and 11.0 using 3% and 4% respectively) under the clear assembled NHS. Two minutes after treatment and after the superficial self-assembling NHS has been removed, it show the extent of cut. 15 minutes after treatment the scale bars = 1mm,