Genomic Phenotyping

We have generated a genomic phenotyping database identifying hundreds of S. cerevisiae genes important for viable cellular recovery after mutagen exposure. Systematic phenotyping of 1,615 gene deletion strains produced distinctive signatures for each of four mutagens. Computational integration of the database with 4,025 interacting proteins, comprising the yeast interactome, identified several multi-protein networks important for damage recovery. Some networks were associated with DNA metabolism and cell cycle control functions, but most were associated with unexpected functions such as cytoskeleton remodeling, chromatin remodeling, and protein, RNA and lipid metabolism. Hence, a plethora of responses other than the DNA damage response are important for recovery.